Part:BBa_K2888006:Design

Chimerical gene (lysozyme and mlrA)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1288
  Illegal AgeI site found at 823
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We add softer linker between two original gene sequence and replace unexpected restriction sites with corresponding code

Source

The mlrA part is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1 and the cyanophage lysozyme part was discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb}